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To investigate the chemical constituents of the rhizome of Arisaema japonicum and study their anti-inflammatory effects. The constituents were isolated by silica gel column chromatography and their structures were identified by spectral analysis (MS, 1H-NMR and 13C-NMR). Six compounds were isolated from the rhizome of Arisaema japonicum and identified as beta-sitosterol (1), stigmasterol (2), 5, 7-dihydroxy-4-O-beta-D-glucopyranosyl flavanone (3), 5-hydroxy-7-methoxy-2, 3-dimethylchroman-4-one (4), genkwanin (5) and genkwanin-5-O-beta-D-glucopyranoside (6). Compounds 1-6 were isolated from the genus Arisaema for the first time. Compound 1 showed significant anti-inflammatory activity against xylene-induced ear oedema in mice at the dose of 0.5 mg.kg-1. Compounds 3 and 6 showed significant anti-inflammatory activity against xylene-induced ear oedema in mice at the dose of 10 mg.kg-1.H 0b46394aab